Identification of a Recombinant Human Interleukin-12 (rhIL-12) Fragment in Non-Reduced SDS-PAGE

Identification of a Recombinant Human Interleukin-12 (rhIL-12) Fragment in Non-Reduced SDS-PAGE

Blog Article

During the past two decades, recombinant human interleukin-12 (rhIL-12) has emerged as one of the most potent cytokines in mediating antitumor activity in a variety of Custom Tote Bag preclinical models and clinical studies.Purity is a critical quality attribute (CQA) in the quality control system of rhIL-12.In our study, rhIL-12 bulks from manufacturer B showed a different pattern in non-reduced SDS-PAGE compared with size-exclusion chromatography (SEC)-HPLC.

A small fragment was only detected in non-reduced SDS-PAGE but not in SEC-HPLC.The results of UPLC/MS and N-terminal sequencing confirmed that the small fragment was a 261–306 amino acid sequence of a p40 subunit of IL-12.The cleavage occurs between Lys260 and Arg261, a basic rich region.

With the presence of 0.2% SDS, the small fragment appeared in both native Servo PAGE and in SEC-HPLC, suggesting that it is bound to the remaining part of the IL-12 non-covalently, and is dissociated in a denatured environment.The results of a bioassay showed that the fractured rhIL-12 proteins had deficient biological activity.

These findings provide an important reference for the quality control of the production process and the final products of rhIL-12.